Gel Electrophoresis Buffers and Diluents
LifeSensors UBITEST BUFFERS ONLY
The UbiTest kit identifies whether a target protein is polyubiquitinated and improves on traditional immunoprecipitation and Western blot methods, which often fail due to epitope masking, reduced antibody affinity, or changes in selectivity. By combining immunoprecipitation with broad‑spectrum DUB digestion, the assay reveals increased signal for the protein of interest after DUB treatment, confirming ubiquitination even when untreated samples show weak detection. To avoid variability caused by antibody–substrate interactions, UbiTest uses high‑affinity TUBEs to pull down polyubiquitinated proteins. These engineered ubiquitin‑binding domains have nanomolar affinity and TUBE1 binds all eight linkage types. The kit contains only the buffers needed for efficient elution of polyubiquitinated proteins.
Hach Company Lysis Buffer Concentrate for SARS-CoV-2 analysis in wastewater using the LuminUltra GeneCount Q-16 qPCR instrument
Lysis Buffer Concentrate for SARS-CoV-2 analysis in wastewater using the LuminUltra GeneCount Q-16 qPCR instrument
Boston Bioproducts Inc qPCR DNase Buffer (10X) - 100ml
qPCR DNase Buffer (10X) - 100ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Separating (Resolving) Buffer - 500ml
The Separating (Resolving) Buffer is a critical component in SDS-Polyacrylamide Gel Electrophoresis (PAGE), a laboratory technique that is used to separate protein molecules based on molecular weight. This buffer maintains the pH of the separating or resolving gel at 8.8, which is a higher pH level than that of the stacking gel.
BIOGEMS INTERNATIONAL INC CFSE 500 ug
CFSE is a reagent useful for cell tracking, proliferation studies, and cell motility studies. After passively diffusing into cells, it is converted to a fluorescent carboxyfluorescin molecule as its acetate groups are cleaved by intracellular esterases. The fluorescent CFSE is retained inside the cell for extended periods, because the molecules forms stable covalent crosslinks with the intracellular proteins. At each cell division, the intensity of the CFSE fluorescence is halved. The peak excitation of the molecule is 494 nm and the peak emission is 521 nm and it may be used with standard fixatives containing formaldehyde and permeabilization buffers that contain saponin.